Synthesis of di ( 2-ethylhexyl) thiophosphoric acid 二(2-乙基己基)硫代磷酸的合成
METHODS: The thiophosphoric acid modified oligonucleotides were transferred into rat peritoneal macrophages directly, TNF content in LPS-stimulated cell culture supernatant was determined by ELISA. 方法:直接将经硫代磷酸修饰的寡核苷酸导入大鼠腹腔巨噬细胞,以减少细胞分泌产生肿瘤坏死因子(TNF)的效果作为判断指标,以ELISA法检测细胞培养上清液中的TNF-α含量。